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Image Search Results
Journal: Scientific Reports
Article Title: Digital pathology with artificial intelligence analysis provides insight to the efficacy of anti-fibrotic compounds in human 3D MASH model
doi: 10.1038/s41598-024-55438-2
Figure Lengend Snippet: Overview of FibroNest™ platform workflow for fibrosis quantification and MASH induction method. ( A ) FibroNest™ quantitative image analysis workflow and method. ( B ) Scheme for MASH induction in hLiMTs. Disease induction and treatment start at day 0 after the production of the hLiMTs. The three treatment conditions are: LEAN, MASH and MASH in the presence of test compounds. After each medium exchange on days 0, 3, 5 and 7, the compounds and vehicle controls (DMSO or PBS) were applied. FFAs supplementation occurred on each treatment day, while stimulation with LPS took place only once during the 10-day treatment period. Cell culture supernatants and/or microtissues were collected on days 5, 7 and 10. All the end points related to pro-collagen type I/III (pro-coll I/III), Histology/SR, TIMPs/matrix metalloproteinases (MMPs) and RNA sequencing (RNA-Seq) were performed on day 10. All conditions were normalized to 0.2% DMSO or PBS.
Article Snippet: The gene expression data were then analyzed with the
Techniques: Cell Culture, RNA Sequencing Assay